We have developed biocompatible polymeric materials which have a repellent property of protein and cell and applying for medical devices such as biosensors, artificial organs, and cell culture devices. For the development of biomaterials in medical devices, study on biointerface between biomolecules such as protein and cell, and biomaterials such as metal, ceramic, and polymer is significant. Because the protein adsorption on material surfaces is the first event when medical devices are in contact with blood. How to evaluate the biointerface?
In this seminar, firstly a mechanism of protein adsorption and cell adhesion on material surfaces is reviewed considering blood coagulation and thrombus formation. And then surface analytical methods of properties of the material surface, adsorption of proteins, and adhesion of cells are explained.
Our experimental results about ΔD-ΔF plots of proteins and cells on the surface with different chemical characteristics (-CH3, -COOH, -NH2, -OH) by QCM-D showed the interaction forces of the biointerface. The interaction analysis of QCM-D was a better understanding with the use of imaging analysis of microscopy.
From these results, we believe that QCM-D is a powerful tool not only for analyzing the amount of protein adsorption on materials, but also for evaluation of an interaction between biomolecules and material surfaces.